Where do mRNA vaccines go? (from Chemist Skeptic)
There has been a lot of discussion about what happens to the mRNA vaccine (like Pfizer and Moderna) once it's injected? This well-written and documented piece by Skeptical Chemist gives us a very clear idea.
Enjoy the reading.
The common notion among professionals is "in the liver", if administered orally (ie orally ) or intravenously, in the liver and on site if injected Intramuscularly.
This 2015 work ( https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4624045/ ) experimentally investigated this with a literally brilliant technique. What was dosed was mRNA that encoded a luciferase ( https://it.wikipedia.org/wiki/Luciferase ). Then luciferine was injected ( https://it.wikipedia.org/wiki/Luciferine ). Tissues that would have expressed luciferase, that is, those where mRNA had arrived, would have "lit" with a green luminescence (I will confess that I am a fan of luminescence-based models). The results: intravenous or intraperitoneal administration "switched on" the liver (which "switched off" in three days). Intramuscular administration the same, but with good luminescence at the injection site (which persisted for one week). With subcutaneous and intradermal injections, however, everything remained at the injection site.
There were people from Acuitas among the authors of this paper, and Acuitas is "in" the Pfizer-Biontech vaccine with its lipid encapsulation technology. The other works cited are by the Moderna and Curevac groups: all the protagonists of mRNA vaccines present and in development have been working on the technology for years.
So where do Pfizer and Moderna mRNA vaccines go? In the muscle at the injection site and in the liver, where they last a maximum of a couple of days (but also in the lymphatic tissue near the injection site and in the spleen).
What, among other things, do we get from all this? That assayed RNA is not back-transcribed and incorporated, as some undaunted continue to argue. The mice did not integrate a luciferase gene in their DNA: when RNA is degraded, the various areas stop glowing. And the same duration of the effect is found in macaques.
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The article Where do mRNA vaccines go? (from Chimicoscettico) comes from ScenariEconomici.it .
This is a machine translation of a post published on Scenari Economici at the URL https://scenarieconomici.it/dove-vanno-a-finire-i-vaccini-rna-da-chimicoscettico/ on Tue, 26 Jan 2021 12:23:32 +0000.